Integrated Mass Spectrometry

Overview of Services

 

The Integrated Mass Spectrometry Shared Resource (IMS-SR) provides high-quality mass analysis of biomolecules – proteins, glycoproteins, peptides, oligonucleotides, nucleic acids, lipids and small-molecule metabolites – for a variety of biomedical investigations. The facility offers supporting techniques as well as scientific consultation.

Notable capabilities of our facility include:

  • Purified proteins and protein complexes (isolated by affinity chromatography) can be rapidly identified by fully-automated liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses combined with our protein database search capabilities.
     
  • Relative protein expression levels can be determined for lysates of whole cells or subcellular organelles as well as paraffin embedded tissue sections.
     
  • Absolute levels of specific peptide components in complex biological samples can be determined (with appropriate stable isotope-labeled standards) via multidimensional separations and MS analysis.
     
  • High-resolution mass spectrometry using electrospray ionization (ESI) provides highly accurate determination of small molecule elemental composition.
     
  • Qualitative and quantitative analysis of small molecules by LCMS/MS.
     
  • High Resolution MS and MSn
     
  • High-resolution mass spectrometry of small molecules and peptides, and complete sequence confirmation for synthetic peptides, can be carried out under GMP conditions. The site is FDA registered. All GMP work requires Quality and Service Agreements PRIOR to analysis. Please contact the facility manager for information regarding agreements.

Email us for more information about Mass Spectrometry or to schedule an appointment for services. A completed submission form must accompany all samples submitted for analysis.

 

Location and hours of operation

 

Hours Location

Monday - Friday

9:00 am to 5:00 pm

  

City of Hope and Beckman Research Institute

1500 East Duarte Road

Hilton Building

Rooms H134, H132, H105

Duarte, CA 91010-3000

 

 

Contacts

Name Role Phone Email Location
Patrick Pirrotte
Director
 
480-236-9510
 
ppirrotte@tgen.org
 
Room H105, Hilton Building
 
Markus Kalkum
Deputy Director
 
626-218-2601
 
mkalkum@coh.org
 
Room H140, Hilton Building
 
Denise Keen
Staff Scientist
 
626-218-2601; 83406
 
dkeen@coh.org
 
Room H132, Hilton Building
 
Roger Moore
Staff Scientist
 
480-236-9510; 83406
 
rmoore@coh.org
 
Room H132, Hilton Building
 
Kate Dabrowska
Research Assistant II
 
480-236-9510; 83406
 
kdabrowska@coh.org
 
Room H132, H105, Hilton Building