The Electron Microscopy and Atomic Force Microscopy Core is available to investigators wishing to define ultrastructural details within their experimental systems. The EM Core personnel assists, as needed, with all aspects of studies brought to the lab. In addition to training on the instruments, guidance is provided, as needed, for experimental design and data interpretation.
Microscopy Services:
Serial block face scanning electron microscopy (SBFSEM)
Scanning transmission electron microscopy (STEM)
Energy-dispersive X-ray spectroscopy (EDS) for elemental analysis of SEM and STEM samples
Transmission electron microscopy (TEM) in conventional, tomographic and cryo-modes
Field emission scanning electron microscopy (FESEM) in secondary electron, backscatter electron and environmental (ESEM) modes
Scanning electron microscopy (SEM)
Fast scanning atomic force microscopy (AFM) in various modes including: contact and non-contact atomic force, lateral force, ScanAsyst (peak force tapping), tapping in air and fluid, magnetic force, and electric force modes
Preparative Services:
Preparations for SBFSEM using the Deerinck et al OTO method
Conventional chemical fixation, resin embedding and ultrathin sectioning for conventional and tomographic TEM
Negative staining for TEM
Cryo-specimen preparation for cryo-TEM
High pressure freezing/freeze substitution combined with embedding in Lowicryl
Post-embedding immunogold labeling on ultrathin Lowicryl sections
Pre-embedding immunogold labeling of vibratome sectioning with gold enhancement
Specimen preparation for scanning electron microscopy including fixation, critical point drying and sputter coating
Sample preparation for atomic force microscopy including spin coating and the preparation of selectively charged surfaces
Link to EM Core Website
Zhuo Li, PhD, Director
zhuoli@coh.org
Hours | Location |
9:00am to 5:00pm Monday through Friday
Extra hours only by special arrangement. |
Furth F1118
Furth F1134
Furth F1135
|
Zhuo Li, PhD, Associate Research Professor, Director
Ricardo Zerda, Staff Scientist
Arlynn Gutierrez, Research Laboratory Assistant
Marcia M. Miller, PhD, Professor, Director Emeritus
Name | Role | Phone | Location | |
---|---|---|---|---|
Zhuo Li, PhD |
Director
|
626 218-8265
|
zhuoli@coh.org
|
Furth F1118/F1134/F1135
|
Ricardo Zerda |
Staff Scientist
|
626 218-8265
|
rzerda@coh.org
|
Furth F1118/F1134/F1135
|