Analytical Pharmacology Core Facility (APCF)

 

The Analytical Pharmacology Core facility (APCF) encourages and facilitiates collaborative research between basic scientists and clinicians by providing a wide range of analytical services that benefit both groups. The APCF conducts pharmacokinetic and pharmacodynamic studies for both chemotherapy clinical trials and peer-reviewed preclinical studies.

 

The primary services of the APCF are:
 

 

Biochemistry Projects
 

The following is a list of possible services associated with the Biochemistry Project:

• ELISA for the quantification of cytokine/chemokine/growth factors in cell culture, plasma or serum samples
• Luminex X-MAP bead array-based to perform simultaneous quantification of multiple cytokine/chemokine/growth factors in plasma, serum, and tissue samples
• Luminex X-MAP bead array to perform simultaneous quantification of phosphorylation status of intracellular signal transduction molecules and transcription factors
• Luminex X-MAP bead array-based to perform RNA Quantigene multiplex assay

 

Cellular Projects
 

The following is a list of possible services associated with the Cellular Project:

• Blood processing, PBMC/serum/plasma isolation, freezing, and storage
• CD8 and NK cytolysis bioassays
• Cell proliferation assays
• In-vitro CD8 and CD4 T cell culture

 

Flow Cytometry Projects
 

The following is a list of possible services associated with the Flow Cytometry Project:

• Absolute count determination and quantification of immune cell subsets
• Analysis of immune cell effector phenotypes
• Characterization of immune cell activation/differentiation/homing status
• Intracellular cytokine staining
• MHC tetramer analysis
• Serological detection of antibodies

 Molecular Projects
 

The following is a list of possible services associated with the Molecular Project:

• cDNA synthesis
• Nucleic acid (DNA, RNA) isolation
• Q-RT-PCR-based quantification of immune cell subsets (including Treg cells)
• Q-RT-PCR-based quantification of mRNA levels for immune effector genes
• Q-PCR-based quantification of adoptively transferred cells

 

The facility’s services are available to both City of Hope and external researchers.