Transgenic / Knockout Animal Program

Overview of Services

The TKAP has been in operation at COH since the early 1990s, and is focussed on the production of genetically-modified animal models for basic and translational research. The TKAP produces transgenic mice by pronuclear microinjection, or by sub-zonal injection of pseudotyped self-inactivating lentiviral vectors. The TKAP generates mouse models carrying targeted gene inactivation (knock-out), or specific modifications of the nucleotide sequence of genes (knock-in) using mouse embronic stem cells (mESC). The TKAP also performs genome engineering with sequence-specific nucleases (TALENs & CRISPR/Cas9) in mESC, or directly in mouse zygotes. In addition the TKAP cryopreserves mouse embryos and gametes, and rederives mouse strains by in vitro fertilization and embryo transfer.

Links and Resources

  1. TKAP

Contacts

Name Role Phone Email Location
Walter Tsark
Director
 
84095
 
wtsark@coh.org
 
655 Huntington Dr