Mass Spectrometry and Proteomics Core Facility

Overview of Services

 

The Mass Spectrometry and Proteomics Core provides high-quality mass analysis of biomolecules – proteins, glycoproteins, peptides, oligonucleotides, nucleic acids, lipids and small-molecule metabolites – for a variety of biomedical investigations. The facility offers supporting techniques as well as scientific consultation.

Notable capabilities of our facility include:

  • Purified proteins and protein complexes (isolated by affinity chromatography) can be rapidly identified by fully-automated liquid chromatography-tandem mass spectrometry (LC/MS/MS) analyses combined with our protein database search capabilities.
     
  • Relative protein expression levels can be determined for lysates of whole cells or subcellular organelles as well as paraffin embedded tissue sections.
     
  • Absolute levels of specific peptide components in complex biological samples can be determined (with appropriate stable isotope-labeled standards) via multidimensional separations and MS analysis.
     
  • High-resolution mass spectrometry using electrospray ionization (ESI) provides highly accurate determination of small molecule elemental composition.
     
  • Qualitative and quantitative analysis of small molecules by LCMS/MS including Metabolomic and Lipidomic profiling.
     
  • High Resolution MS and MSn
     
  • High-resolution mass spectrometry of small molecules and peptides, and complete sequence confirmation for synthetic peptides, can be carried out under GMP conditions. The site is FDA registered. All GMP work requires Quality and Service Agreements PRIOR to analysis. Please contact the facility manager for information regarding agreements.

 

Email us for more information about Mass Spectrometry or to schedule an appointment for services or equipment. Please see the Fee Schedule for pricing for various analyses or shared instrument usage. A completed submission form must accompany all samples submitted for analysis.

 

Leadership

 

Markus Kalkum, Core Director

Gabriel Gugiu, Core Manager

Location and hours of operation

 

Hours Location

Monday - Friday

10:00 am to 6:00 pm

(Access is available after hours to trained users by request.)     

City of Hope and Beckman Research Institute

1500 East Duarte Road

Hilton Building

Rooms H134, H132, H105

Duarte, CA 91010-3000

Links and Resources

 

  1. http://www.cityofhope.org/mass-spectrometry-and-proteomics

 

Roger, Helen and Denise:

    

 

 

Contacts

Name Role Phone Email Location
Markus Kalkum
Core Director
 
626-218-2601
 
mkalkum@coh.org
 
Office H140, Hilton Building
 
Gabriel Gugiu
Core Manager
 
626-218-2601; 85905; 88389
 
ggugiu@coh.org
 
Office H105, Hilton Building
 
Roger Moore
Assistant Manager
 
626-218-2601; 83406
 
rmoore@coh.org
 
Room H132, Hilton Building
 
Denise Keen
Staff Scientist
 
626-218-2601; 83406
 
dkeen@coh.org
 
Room H132, Hilton Building
 
Helen Ge
Senior Research Associate
 
626-218-2601; 83406
 
hge@coh.org
 
Room H132, Hilton Building